pcmv ha e2f5 (Addgene inc)
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Addgene inc
pcmv ha e2f5
Pcmv Ha E2f5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+ha+e2f5/pCMVHA-E2F5+(Plasmid+%2324213)/pmc13022133-37-0-3
Average 92 stars, based on 7 article reviews
Pcmv Ha E2f5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+ha+e2f5/pCMVHA-E2F5+(Plasmid+%2324213)/pmc13022133-37-0-3
Average 92 stars, based on 7 article reviews
pcmv ha e2f5 - by Bioz Stars,
2026-10
92/100 stars
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Clone Assay:Article Title: GEMC1 is a critical regulator of multiciliated cell differentiation. Article Snippet: GFP-GEMC1-G313D was generated by site-directed mutagenesis (Quick-Change sitedirected mutagenesis kit (Qiagen)) using pIRESpuro3-GFP-FlagGEMC1 as template and the primer 50-CATTCCTTCCACCAGGACC AAGCCTTTGTGCG-30. .. E2F4 and DP1 cDNAs (Addgene plasmids #10914 and #37968) were cloned into the mammalian expression vector pcDNA3.1-HA (Addgene) at the EcoRI and BamHI restriction sites to produce proteins N-terminally fused to HA under the control of the constitutive CMV promoter. Expressing:Article Title: GEMC1 is a critical regulator of multiciliated cell differentiation. Article Snippet: GFP-GEMC1-G313D was generated by site-directed mutagenesis (Quick-Change sitedirected mutagenesis kit (Qiagen)) using pIRESpuro3-GFP-FlagGEMC1 as template and the primer 50-CATTCCTTCCACCAGGACC AAGCCTTTGTGCG-30. .. E2F4 and DP1 cDNAs (Addgene plasmids #10914 and #37968) were cloned into the mammalian expression vector pcDNA3.1-HA (Addgene) at the EcoRI and BamHI restriction sites to produce proteins N-terminally fused to HA under the control of the constitutive CMV promoter. Plasmid Preparation:Article Title: GEMC1 is a critical regulator of multiciliated cell differentiation. Article Snippet: GFP-GEMC1-G313D was generated by site-directed mutagenesis (Quick-Change sitedirected mutagenesis kit (Qiagen)) using pIRESpuro3-GFP-FlagGEMC1 as template and the primer 50-CATTCCTTCCACCAGGACC AAGCCTTTGTGCG-30. .. E2F4 and DP1 cDNAs (Addgene plasmids #10914 and #37968) were cloned into the mammalian expression vector pcDNA3.1-HA (Addgene) at the EcoRI and BamHI restriction sites to produce proteins N-terminally fused to HA under the control of the constitutive CMV promoter. Control:Article Title: GEMC1 is a critical regulator of multiciliated cell differentiation. Article Snippet: GFP-GEMC1-G313D was generated by site-directed mutagenesis (Quick-Change sitedirected mutagenesis kit (Qiagen)) using pIRESpuro3-GFP-FlagGEMC1 as template and the primer 50-CATTCCTTCCACCAGGACC AAGCCTTTGTGCG-30. .. E2F4 and DP1 cDNAs (Addgene plasmids #10914 and #37968) were cloned into the mammalian expression vector pcDNA3.1-HA (Addgene) at the EcoRI and BamHI restriction sites to produce proteins N-terminally fused to HA under the control of the constitutive CMV promoter. other:Article Title: GemC1 governs multiciliogenesis through direct interaction with and transcriptional regulation of p73. Article Snippet: Protein samples were then analyzed with SDS-PAGE, transferred on PVDF membrane (Merck Millipore / IPVH00010) followed by Western blot analysis. Article Title: McIdas localizes to centrioles and controls centriole numbers through PLK4-dependent phosphorylation Article Snippet: |